Metabolomics analysis reveals 6‐benzylaminopurine as a stimulator for improving lipid and DHA accumulation of Aurantiochytrium sp.

作者：Xin‐Jun Yu, Jie Sun, Jianyong Zheng, Yaqi Sun, Zhao Wang · 发表于：Journal of Chemical Technology & Biotechnology · 年份：2015 · DOI：10.1002/jctb.4869 · 被引用次数：53 · 研究领域：Algal biology and biofuel production、Seaweed-derived Bioactive Compounds、Microbial Metabolic Engineering and Bioproduction

Abstract BACKGROUND Phytohormones are chemical messengers that have a positive effect on biodiesel production of microalgae at low concentrations. However, the effect of phytohormone 6‐benzylaminopurine on lipid and docosahexaenoic acid ( DHA ) production in marine DHA ‐producer Aurantiochytrium has never been reported. In this study, a GC‐MS ‐based metabolomics method combined with a multivariate analysis is applied to reveal the metabolic mechanism of 6‐benzylaminopurine enhancing production of lipid and DHA in Aurantiochytrium sp. YLH70 . RESULTS In total, 71 metabolites were identified by GC‐MS . The PCA model revealed that 76.9% of metabolite variation was related to 6‐benzylaminopurine treatment, and overall metabolomics profiles between the 6‐benzylaminopurine and control groups were clearly discriminated. Forty‐six metabolites identified by the PLS‐DA model were responsible for responding to 6‐benzylaminopurine. Metabolic analysis showed that 6‐benzylaminopurine could accelerate the rate of utilization of glucose in Aurantiochytrium sp. YLH70 , and the metabolic flux from glycolysis, TCA cycle and mevalonate pathway to fatty acids biosynthesis was promoted. Moreover, the anti‐stress mechanism in Aurantiochytrium sp. YLH70 might be induced by 6‐benzylaminopurine. CONCLUSION Metabolomics is a suitable tool to discover the metabolic mechanism for improving lipid and DHA accumulation in a microorganism. 6‐benzylaminopurine has the potential to stimulate lipid and DHA prod...