Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies

作者：Harry B. Greenberg, V. J. McAuliffe, J Valdesuso, Robert J. Wyatt, Jorge Flores, Anthony R. Kalica, Yasutaka Hoshino, Namita Singh · 发表于：Infection and Immunity · 年份：1983 · DOI：10.1128/iai.39.1.91-99.1983 · 被引用次数：358 · 研究领域：Viral gastroenteritis research and epidemiology、Animal Virus Infections Studies、Vector-Borne Animal Diseases

Ten monoclones directed to the 42,000-dalton inner structural protein of rotavirus were analyzed. Eight monoclones reacted broadly with antigenic domains common to virtually all mammalian rotaviruses. Two monoclones had specificities similar or identical to previously characterized subgroup specificities. These subgroup monoclones were more efficient in detecting subgroup antigen than either hyperimmune or postinfection antisera. Using the subgroup monoclones, we determined that some animal as well as human rotavirus strains carry subgroup 2 specificity and that epizootic diarrhea of infant mice virus and turkey rotavirus are antigenically distinct from other mammalian rotavirus strains.