Hydrogen Peroxide Modulates Electrophysiological Characteristics of Left Atrial Myocytes.

作者：Shih Yu Huang, Yen‐Yu Lu, Yao Chang Chen, Wei Ta Chen, Yung Kuo Lin, Shih Ann Chen, Yi Jen Chen · 发表于：PubMed · 年份：2014 · 被引用次数：9 · 研究领域：Cardiac electrophysiology and arrhythmias、Cardiac Ischemia and Reperfusion、Microbial Inactivation Methods

BACKGROUND: Oxidative stress plays an important role in the pathophysiology of atrial fibrillation (AF). The hydrogen peroxide (H2O2) mainly underlies the cellular oxidative stress and free radicals. Left atrium (LA) is the most important AF substrate. However, the effects of H2O2 on the action potential (AP) and ionic currents in LA myocytes have not been fully elucidated. METHODS: The whole-cell patch clamp was used to investigate the APs and ionic currents of L-type calcium current (ICa-L), transient outward currents (Ito), ultra-rapid delayed rectifier potassium current (IKur), delayed rectifier potassium currents (IK), inward rectifier potassium current (IK1), and sodium-calcium exchanger (NCX) before and after H2O2 (100 μM) in isolated rabbit LA myocytes. RESULTS: H2O2 (100 μM) shortened by 50% (from 40 ± 7 to 21 ± 5 ms) and 90% the AP duration (from 95 ± 12 to 74 ± 11 ms) in LA myocytes (n = 9), but did not change the resting membrane potentials. The H2O2 (100 μM) decreased Ito, but increased IKur and IK. H2O2 (100 μM) also reduced the ICa-L and the reverse mode NCX. However, H2O2 (100 μM) did not change IK1. CONCLUSIONS: H2O2 directly modulated the AP morphology and ionic currents in LA myocytes, which may contribute to the genesis of AF in oxidative stress. KEY WORDS: Action potential; Ionic current; Oxidative stress.