The fission yeast cdc19+ gene encodes a member of the MCM family of replication proteins

作者：Susan L. Forsburg, Paul Nurse · 发表于：Journal of Cell Science · 年份：1994 · DOI：10.1242/jcs.107.10.2779 · 被引用次数：76 · 研究领域：DNA Repair Mechanisms、Fungal and yeast genetics research、Genomics and Chromatin Dynamics

We have cloned and characterized the fission yeast cdc19+ gene. We demonstrate that it encodes a structural homologue of the budding yeast MCM2 protein. In fission yeast, the cdc19+ gene is constitutively expressed, and essential for viability. Deletion delays progression through S phase, and cells arrest in the first cycle with an apparent 2C DNA content, with their checkpoint control intact. The temperature-sensitive cdc19-P1 mutation is synthetically lethal with cdc21-M68. In addition, we show by classical and molecular genetics that cdc19+ is allelic to the nda1+ locus. We conclude that cdc19p plays a potentially conserved role in S phase.