Identification of Serum Biomarkers for Gastric Cancer Diagnosis Using a Human Proteome Microarray

作者：Lina Yang, Jingfang Wang, Jianfang Li, Hainan Zhang, Shujuan Guo, Min Yan, Zhenggang Zhu, Bin Lan, Youcheng Ding, Ming Xu, Wei Li, Xiaonian Gu, Qi Chong, Heng Zhu, Zhifeng Shao, Bingya Liu, Sheng‐ce Tao · 发表于：Molecular & Cellular Proteomics · 年份：2015 · DOI：10.1074/mcp.m115.051250 · 被引用次数：96 · 研究领域：Gene expression and cancer classification、Advanced Biosensing Techniques and Applications、Advanced Proteomics Techniques and Applications

We aimed to globally discover serum biomarkers for diagnosis of gastric cancer (GC). GC serum autoantibodies were discovered and validated using serum samples from independent patient cohorts encompassing 1,401 participants divided into three groups, i.e. healthy, GC patients, and GC-related disease group. To discover biomarkers for GC, the human proteome microarray was first applied to screen specific autoantibodies in a total of 87 serum samples from GC patients and healthy controls. Potential biomarkers were identified via a statistical analysis protocol. Targeted protein microarrays with only the potential biomarkers were constructed and used to validate the candidate biomarkers using 914 samples. To provide further validation, the abundance of autoantibodies specific to the biomarker candidates was analyzed using enzyme-linked immunosorbent assays. Receiver operating characteristic curves were generated to evaluate the diagnostic accuracy of the serum biomarkers. Finally, the efficacy of prognosis efficacy of the final four biomarkers was evaluated by analyzing the clinical records. The final panel of biomarkers consisting of COPS2, CTSF, NT5E, and TERF1 provides high diagnostic power, with 95% sensitivity and 92% specificity to differentiate GC patients from healthy individuals. Prognosis analysis showed that the panel could also serve as independent predictors of the overall GC patient survival. The panel of four serum biomarkers (COPS2, CTSF, NT5E, and TERF1) could se...