Expression of Hailan chicken MyoD in fibroblast.

作者：Hongyan Wang, Hu Lan, Tingting Zhang, Yuan Yaqi, Wenbo Hao, Xiaoyu Yang · 年份：2010 · 研究领域：Muscle Physiology and Disorders

MyoD cDNA was amplified through reverse transfer polymerase chain reaction(RT-PCR),and then cloned and sequenced.The eukaryotic expression vector pEGFP-N1-MyoD was constructed,and was transfected into chick embryo fibroblast with liposome.The MyoD protein encoded by this gene was detected respectively with fluorescence microscope,RT-PCR and SDS-PAGE.The results showed that eukaryotic expression vector pEGFP-N1-MyoD was transfected into chick embryo fibroblast successfully.The green fluorescence was observed under a fluorescence microscope,the objective band was detected with RT-PCR and the MyoD protein was detected with SDS-PAGE,which was about 33KD.The results provided experimental basis for the function of MyoD protein.