Elevated miR-155 expression induces immunosuppression via CD39 + regulatory T-cells in sepsis patient

作者：Jingquan Liu, Kai Shi, Minhua Chen, Liang Xu, Jun Hong, Bangchuan Hu, Xianghong Yang, Ren-Hua Sun · 发表于：International Journal of Infectious Diseases · 年份：2015 · DOI：10.1016/j.ijid.2015.09.016 · 被引用次数：67 · 研究领域：MicroRNA in disease regulation、Inflammation biomarkers and pathways、Immune Cell Function and Interaction

BACKGROUND: An altered microRNA profile exists in many infectious diseases, including sepsis. CD39(+) regulatory T-cells (Tregs) have a remarkable immunosuppressive effect and play an important role in the regulation of immune balance in sepsis. However, the correlation between microRNA changes and the ratio of CD39(+) Tregs in sepsis patients has not yet been reported. The altered microRNA expression profile in sepsis patients was analyzed in this study. Moreover, the correlation between microRNAs and disease severity and prognosis was investigated. Furthermore, the correlation between microRNAs and the percentage of peripheral blood CD39(+) Tregs was investigated and further verified in an animal model. METHODS: Sixty sepsis patients and 30 healthy controls were included. The difference in microRNA expression was investigated by microRNA microarray and was further confirmed by real-time quantitative PCR. The correlations between microRNA changes and the Sepsis-related Organ Failure Assessment (SOFA) score, severity of sepsis, and survival were analyzed. The percentage CD39(+) Tregs in the peripheral blood of sepsis patients was measured by flow cytometry. The correlation between microRNAs and the percentage CD39(+) Tregs was analyzed and further confirmed in a mouse sepsis model. RESULTS: Compared to healthy controls, sepsis patients exhibited a significantly elevated microRNA-155 (miR-155) level (p < 0.05), which was positively related to a higher SOFA score (r = 0.641, p ...