Application of a Collagen-Based Membrane and Chondroitin Sulfate-Based Hydrogel Adhesive for the Potential Repair of Severe Ocular Surface Injuries

作者：Je-Min Chae, Daniel G. Mulreany, Qiongyu Guo, Qiaozhi Lu, Joseph Choi, Iossif Strehin, Freddy A. Espinoza, Oliver D. Schein, Morgana M. Trexler, Kraig S. Bower, Jennifer H. Elisseeff · 发表于：Military Medicine · 年份：2014 · DOI：10.7205/milmed-d-13-00360 · 被引用次数：24 · 研究领域：Corneal Surgery and Treatments、Intraocular Surgery and Lenses、Ocular Surface and Contact Lens

This study was performed to evaluate the potential of a chondroitin sulfate-polyethylene glycol (CS-PEG) adhesive and collagen-based membrane (collagen vitrigel, CV) combination as a method to treat penetrating ocular injuries on the battlefield and to improve this method with two technologies: an antibiotic releasing CS-PEG adhesive and a corneal shaped CV. Burst testing using porcine cadaveric eyes, high-performance liquid chromatography, the Kirby-Bauer bacterial inhibition test, and CV implantations on the live and cadaveric rabbit eyes were performed. The ocular burst test showed CS-PEG adhesive could successfully repair 5-mm to 6-mm length wounds in the corneal and corneoscleral regions but would require CS-PEG + CV to treat larger wounds similar to those seen on the battlefield. In addition, high performance liquid chromatography and the Kirby-Bauer bacterial inhibition test presented evidence suggesting the vancomycin incorporated CS-PEG could inhibit Staphylococcus infection for 9 days. Furthermore, the curved CV showed an advantage by matching the corneal contour without any wrinkle formation. Although this pilot study showed a limited range of possible applications, we demonstrated that the combination of CS-PEG adhesive + CV is a promising method and the 2 technologies improve their applicability to the special demands of the battlefield.