Method of birth alters interferon‐gamma and interleukin‐12 production by cord blood mononuclear cells

作者：Mark A. Brown, Parmis Y. Rad, Marilyn J. Halonen · 发表于：Pediatric Allergy and Immunology · 年份：2003 · DOI：10.1034/j.1399-3038.2003.00015.x · 被引用次数：32 · 研究领域：Immune Cell Function and Interaction、Immune Response and Inflammation、Hematopoietic Stem Cell Transplantation

Many uncertainties exist regarding the capability of cord blood mononuclear cells (CBMC) to produce cytokines. A number of conflicting reports led us to examine the effects of method of birth on CBMC production of interferon-gamma (IFN-gamma), interleukin-4 (IL-4) and interleukin-12 (IL-12). While constitutive production of IL-4 was found in both vaginally and cesarean-delivered infants, constitutive IFN-gamma or IL-12 production was found in neither. CBMC from vaginally delivered infants responded to stimulation with concanavalin A/phorbol 12-myristate 13-acetate (Con A/PMA), phytohemagglutinin (PHA), and lipopolysaccharide (LPS) with significantly higher levels of IFN-gamma than CBMC from unlabored cesarean section (CS) infants. Production of IL-12 was increased in the vaginally delivered group in response to LPS and PHA but not to ConA/PMA. In contrast, mode of delivery was not associated with differences in IL-4 production. These results indicate that mode of delivery significantly alters the capability of CBMC to produce some cytokines and therefore should be taken into account in interpreting fetal/neonatal mononuclear cell function studies.