Characterization of a Purified Photosystem II-Phycobilisome Particle Preparation from Porphyridium cruentum

作者：Barbara M. Chereskin, Jenny D. Clément‐Métral, Elisabeth Gantt · 发表于：PLANT PHYSIOLOGY · 年份：1985 · DOI：10.1104/pp.77.3.626 · 被引用次数：27 · 研究领域：Photosynthetic Processes and Mechanisms、Algal biology and biofuel production、Microbial Community Ecology and Physiology

Detergent preparations isolated from thylakoids of the red alga Porphyridium cruentum, in a sucrose, phosphate, citrate, magnesium chloride medium consist of phycobilisomes and possess high rates of photosystem II activity. Characterization of these particles shows that the O(2)-evolving activity is stable for several hours and the pH optimum is about 6.5 to 7.2. Response of the system to light, electron donors and acceptors, and inhibitors verify that the observed activity, measured both as O(2) evolution and 2,6-dichlorophenol-indophenol reduction, is due to photosystem II. Furthermore, photosystem II is functionally coupled to the phycobilisome in this preparation since green light, absorbed by phycobilisomes of P. cruentum, is effective in promoting both O(2) evolution and 2,6-dichlorophenol-indophenol reduction. Photosystem II activity declines when light with wavelengths shorter than 665 nm is removed. Both 3-(3,4-dichlorophenyl)-1,1-dimethylurea and atrazine inhibit photosystem II activity in this preparation, indicating that the herbicide binding site is a component of the photosystem II-phycobilisome particle.