High-Throughput Proteomics

作者：Zhaorui Zhang, Si Wu, David L. Stenoien, Ljiljana Paša‐Tolić · 发表于：Annual Review of Analytical Chemistry · 年份：2014 · DOI：10.1146/annurev-anchem-071213-020216 · 被引用次数：330 · 研究领域：Advanced Proteomics Techniques and Applications、Mass Spectrometry Techniques and Applications、Metabolomics and Mass Spectrometry Studies

Mass spectrometry (MS)-based high-throughput proteomics is the core technique for large-scale protein characterization. Due to the extreme complexity of proteomes, sophisticated separation techniques and advanced MS instrumentation have been developed to extend coverage and enhance dynamic range and sensitivity. In this review, we discuss the separation and prefractionation techniques applied for large-scale analysis in both bottom-up (i.e., peptide-level) and top-down (i.e., protein-level) proteomics. Different approaches for quantifying peptides or intact proteins, including label-free and stable-isotope-labeling strategies, are also discussed. In addition, we present a brief overview of different types of mass analyzers and fragmentation techniques as well as selected emerging techniques.