Gene-expression profiling of human osteoblasts following treatment with the ionic products of Bioglass� 45S5 dissolution

作者：Ioannis D. Xynos, Alasdair John Edgar, Lee D.K. Buttery, Larry L. Hench, Julia Margaret Polak · 发表于：Journal of Biomedical Materials Research · 年份：2001 · DOI：10.1002/1097-4636(200105)55:2<151::aid-jbm1001>3.0.co;2-d · 被引用次数：1222 · 研究领域：Bone Tissue Engineering Materials、Bone and Dental Protein Studies、Bone Metabolism and Diseases

The effect of the ionic products of Bioglass 45S5 dissolution on the gene-expression profile of human osteoblasts was investigated by cDNA microarray analysis of 1,176 genes. Treatment with the ionic products of Bioglass 45S5 dissolution increased the levels of 60 transcripts twofold or more and reduced the levels of five transcripts to one-half or less than in control. Markedly up-regulated genes included RCL, a c-myc responsive growth related gene, cell cycle regulators such as G1/S specific cyclin D1, and apoptosis regulators including calpain and defender against cell death (DAD1). Other significantly up-regulated genes included the cell surface receptors CD44 and integrin beta1, and various extracellular matrix regulators including metalloproteinases-2 and -4 and their inhibitors TIMP-1 and TIMP-2. The identification of differentially expressed genes by cDNA microarray analysis has offered new insights into the mode of action of bioactive glasses and has proven to be an effective tool in evaluating their osteoproductive properties.