The v-myb oncogene product binds to and activates the promyelocyte-specific mim-1 gene

作者：Scott A. Ness, Åsa Marknell, Thomas Graf · 发表于：Cell · 年份：1989 · DOI：10.1016/0092-8674(89)90767-8 · 被引用次数：453 · 研究领域：RNA Interference and Gene Delivery、Acute Myeloid Leukemia Research、Drug Transport and Resistance Mechanisms

The v-myb oncogene induces myeloid leukemias in chickens, transforms myeloid cells in vitro, and encodes a sequence-specific DNA binding protein. We used differential hybridization to screen for v-myb-regulated genes in cells transformed by a temperature-sensitive mutant of the oncogene and identified a new gene, mim-1, which encodes a specifically expressed, secretable protein contained in the granules of both normal and v-myb-transformed promyelocytes. The promoter of the mim-1 gene contains three closely spaced binding sites for v-myb protein and is strongly activated by v-myb in a cotransfection assay. Synthetic copies of the binding sites are both necessary and sufficient to confer v-myb protein-dependent activation to a heterologous promoter. We conclude that mim-1 is a cellular gene that is directly regulated by the product of the v-myb oncogene.