EVIDENCE FOR A SINGLE-STRANDED ADENOVIRUS-ASSOCIATED VIRUS GENOME: FORMATION OF A DNA DENSITY HYBRID ON RELEASE OF VIRAL DNA

作者：James A. Rose, Kenneth I. Berns, M. DAVID HOGGAN, Frank J. Koczot · 发表于：Proceedings of the National Academy of Sciences · 年份：1969 · DOI：10.1073/pnas.64.3.863 · 被引用次数：179 · 研究领域：Virus-based gene therapy research、Bacteriophages and microbial interactions、Molecular Biology Techniques and Applications

Extracted adenovirus-associated virus DNA is known to be double-stranded, and, therefore, it has been assumed that these virus particles contain a double-stranded genome. Recent findings, however, have suggested that the DNA in virus particles is equivalent to only half the molecular weight of extracted molecules. A density analysis of DNA extracted from a mixture of virus particles containing either bromodeoxyuridine-substituted or unsubstituted DNA shows that virions contain single-stranded DNA which, when released, forms duplex structures. A similar circumstance is as yet unknown among other viruses.