Comparison of the anti‐apoptotic effects of Bcr‐Abl, Bcl‐2 and Bcl‐xL following diverse apoptogenic stimuli

作者：Gabriela Brumatti, Ricardo Weinlich, Cristina F Chehab, Monica Yon, Gustavo Pessini Amarante-Mendes · 发表于：FEBS Letters · 年份：2003 · DOI：10.1016/s0014-5793(03)00299-0 · 被引用次数：40 · 研究领域：Synthesis and Characterization of Heterocyclic Compounds、Chronic Myeloid Leukemia Treatments、Quinazolinone synthesis and applications

Ectopic expression of Bcr-Abl, Bcl-2 or Bcl-x(L) in HL-60 cells conferred resistance to apoptosis against a variety of death-inducing agents. Bcr-Abl-mediated interference with mitochondrial events was confirmed by the analysis of the loss of mitochondrial transmembrane potential and cytochrome c release. HL-60.Bcr-Abl cells were extremely resistant to all apoptogenic stimuli tested, even in circumstances where HL-60.Bcl-2 or HL-60.Bcl-x(L) cells were only partially protected from apoptosis. The levels of Mcl-1, Bax, Bid, Akt, c-IAP-1, c-IAP-2, XIAP and c-FLIP were compared in all HL-60 lines. Our findings show that Bcr-Abl is a more powerful anti-apoptotic molecule than Bcl-2 or Bcl-x(L).