Isolation and Characterization of a Water Stress-Specific Genomic Gene, pwsi 18, from Rice

作者：Nirmal Joshee, Hiroaki Kisaka, Yoshichika Kitagawa · 发表于：Plant and Cell Physiology · 年份：1998 · DOI：10.1093/oxfordjournals.pcp.a029290 · 被引用次数：47 · 研究领域：Rice Cultivation and Yield Improvement、Plant responses to water stress、Plant Stress Responses and Tolerance

One of the water stress-specific cDNA clones of rice characterised previously, wsi18, was selected for further study. The wsi18 gene can be induced by water stress conditions such as mannitol, NaCl, and dryness, but not by ABA, cold, or heat. A genomic clone for wsi18, pwsi18, contained about 1.7 kbp of the 5' upstream sequence, two introns, and the full coding sequence. The 5'-upstream sequence of pwsi18 contained putative cis-acting elements, namely an ABA-responsive element (ABRE), three G-boxes, three E-boxes, a MEF-2 sequence, four direct and two inverted repeats, and four sequences similar to DRE, which is involved in the dehydration response of Arabidopsis genes. The gusA reporter gene under the control of the pwsi18 promoter showed transient expression in response to water stress. Deletion of the downstream DRE-like sequence between the distal G-boxes-2 and -3 resulted in rather low GUS expression.