Electrochemical Gene‐Function Analysis for Single Cells with Addressable Microelectrode/Microwell Arrays

作者：Zhenyu Lin, Yasufumi Takahashi, Tatsuya Murata, Michiaki Takeda, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue · 发表于：Angewandte Chemie International Edition · 年份：2009 · DOI：10.1002/anie.200805743 · 被引用次数：61 · 研究领域：Neuroscience and Neural Engineering、Advanced biosensing and bioanalysis techniques、Electrochemical Analysis and Applications

To each his own: An addressable electrochemical device consisting of orthogonally arranged rows and columns of electrodes has been constructed to monitor protein expression in genetically engineered cells at the single-cell level. The response based on redox cycling reflected the different expression levels of the enzyme from individual HeLa cells transfected with a plasmid vector including secreted alkaline phosphatase.