Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus

作者：Harry B. Greenberg, J Valdesuso, Kathleen Van Wyke, K Midthun, M.P. Walsh, V. J. McAuliffe, Richard G. Wyatt, Anthony R. Kalica, Jorge Flores, Yasutaka Hoshino · 发表于：Journal of Virology · 年份：1983 · DOI：10.1128/jvi.47.2.267-275.1983 · 被引用次数：287 · 研究领域：Viral gastroenteritis research and epidemiology、Viral Infectious Diseases and Gene Expression in Insects、Virus-based gene therapy research

A series of monoclonal antibodies was isolated which reacted with one of two major surface proteins of rhesus rotavirus. Thirty-six monoclonal antibodies immunoprecipitated the 82-kilodalton outer capsid protein, the product of the fourth gene, the viral hemagglutinin. These monoclonal antibodies exhibited hemagglutination inhibition activity and neutralized rhesus rotavirus to moderate or high titer. Three monoclonal antibodies immunoprecipitated the 38-kilodalton outer capsid glycoprotein, the eighth or ninth gene product. These three monoclonal antibodies neutralized rhesus rotavirus to high titer and also inhibited viral hemagglutination.