Western blot (immunoblot) assay of small, round-structured virus associated with an acute gastroenteritis outbreak in Tokyo

作者：Yujiro Hayashi, Tamie Ando, Etsuko UTAGAWA, Seiji Sekine, S Okada, Kiyoshi Yabuuchi, Takashi Miki, Makoto Ohashi · 发表于：Journal of Clinical Microbiology · 年份：1989 · DOI：10.1128/jcm.27.8.1728-1733.1989 · 被引用次数：49 · 研究领域：Viral gastroenteritis research and epidemiology、Animal Virus Infections Studies、Bacteriophages and microbial interactions

Small, round-structured virus (SRSV) was detected in a stool specimen of a patient during an acute gastroenteritis outbreak in Tokyo and was tentatively named SRSV-9. SRSV-9 was purified by sucrose velocity gradient centrifugation after CsCl density gradient centrifugation. The buoyant density of SRSV-9 appeared to be 1.36 g/ml in CsCl. A Western blot (immunoblot) assay using the biotin-avidin system revealed that SRSV-9 was antigenically related to the Hawaii agent but distinct from the Norwalk agent and contained a single major structural protein with a molecular size of 63.0 +/- 0.6 kilodaltons. The prevalence of SRSV-9 infection in Tokyo was surveyed by the Western blot antibody assay by using a crude virus preparation as the antigen. Seroconversion was observed in 56.5% of the patients involved in the outbreaks from which SRSV was detected by electron microscopy.