Strategies for the Assessment of Protein Aggregates in Pharmaceutical Biotech Product Development

作者：John den Engelsman, Patrick Garidel, Ronald A. Smulders, Hans Koll, Bryan Ronain Smith, Stefan Bassarab, Andreas Seidl, Otmar Hainzl, Wim Jiskoot · 发表于：Pharmaceutical Research · 年份：2010 · DOI：10.1007/s11095-010-0297-1 · 被引用次数：354 · 研究领域：Protein purification and stability、Monoclonal and Polyclonal Antibodies Research、Biosimilars and Bioanalytical Methods

Within the European Immunogenicity Platform (EIP) ( http://www.e-i-p.eu ), the Protein Characterization Subcommittee (EIP-PCS) has been established to discuss and exchange experience of protein characterization in relation to unwanted immunogenicity. In this commentary, we, as representatives of EIP-PCS, review the current state of methods for analysis of protein aggregates. Moreover, we elaborate on why these methods should be used during product development and make recommendations to the biotech community with regard to strategies for their application during the development of protein therapeutics.