Cloning of the cDNA and gene for a human D2 dopamine receptor.

作者：David K. Grandy, Mark A. Marchionni, H Makam, R E Stofko, Massimiliano Alfano, L Frothingham, Jürgen Fischer, Kathy J Burke-Howie, James R. Bunzow, Alfred C. Server · 发表于：Proceedings of the National Academy of Sciences · 年份：1989 · DOI：10.1073/pnas.86.24.9762 · 被引用次数：488 · 研究领域：Monoclonal and Polyclonal Antibodies Research、Chemical Synthesis and Analysis、Click Chemistry and Applications

A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D2 receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.