Interleukin 2 regulates the expression of Tac antigen on peripheral blood T lymphocytes.

作者：Karl Welte, Michael Andreeff, Erich Platzer, Kathryn L. Holloway, Berish Y. Rubin, Michelle A. Moore, Roland H. Mertelsmann · 发表于：The Journal of Experimental Medicine · 年份：1984 · DOI：10.1084/jem.160.5.1390 · 被引用次数：153 · 研究领域：Monoclonal and Polyclonal Antibodies Research、T-cell and B-cell Immunology、Immune Cell Function and Interaction

We investigated the effect of OKT3 antibody and interleukin 2 (IL-2) on Tac antigen expression and the proliferation of human peripheral blood mononuclear leukocytes. OKT3 monoclonal antibody at low, nonmitogenic concentrations (25 pg/ml) or IL-2 alone at optimal concentrations (20 U/ml) did not induce IL-2 receptor expression, as measured by Tac antibody or by T cell proliferation. However, costimulation with these concentrations of OKT3 antibody and IL-2 led to Tac antigen expression and T cell proliferation. These data suggest that the T cells are activated in two steps: OKT3 antibody at 25 pg/ml does not induce Tac antigen expression, but preactivates T cells to become responsive to IL-2. The addition of exogenous IL-2 then leads to expression of the IL-2 receptor, as recognized by Tac antibody, and to subsequent proliferation.