Both mTORC1 and mTORC2 are involved in the regulation of cell adhesion

作者：Long Chen, Baoshan Xu, Lei Liu, Chunxiao Liu, Yan Luo, Xin Chen, Mansoureh Barzegar, Jun Chung, Shile Huang · 发表于：Oncotarget · 年份：2015 · DOI：10.18632/oncotarget.3044 · 被引用次数：84 · 研究领域：PI3K/AKT/mTOR signaling in cancer、Coagulation, Bradykinin, Polyphosphates, and Angioedema、Polyamine Metabolism and Applications

// Long Chen 1, 2 , Baoshan Xu 2 , Lei Liu 2 , Chunxiao Liu 1 , Yan Luo 2 , Xin Chen 2 , Mansoureh Barzegar 2 , Jun Chung 2, 3 , Shile Huang 2, 3 1 Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, P. R. China 2 Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA 3 Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA Correspondence to: Shile Huang, e-mail: shuan1@lsuhsc.edu Long Chen, e-mail: lchen@njnu.edu.cn Keywords: rapamycin, mTOR, cell adhesion, Akt, 4E-BP1 Received: September 09, 2014      Accepted: January 07, 2015      Published: January 23, 2015 ABSTRACT mTOR is a central controller for cell growth/proliferation and survival. Recent studies have shown that mTOR also regulates cell adhesion, yet the underlying mechanism is not known. Here we found that inhibition of mTOR by rapamycin reduced the basal or type I insulin-like growth factor (IGF-1)-stimulated adhesion of cancer cells. Further research revealed that both mTORC1 and mTORC2 were involved in the regulation of cell adhesion, as silencing expression of raptor or rictor inhibited cell adhesion. Also, PP242, an mTORC1/2 kinase inhibitor, inhibited cell adhesion more potently than rapamycin (mTOR...