Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition

作者：Andrew Adey, Hilary G. Morrison, Asan, Xun Xu, Jacob Otto Kitzman, Emily H. Turner, Bethany Stackhouse, Alexandra P MacKenzie, Nicholas C. Caruccio, Xiuqing Zhang, Jay Shendure · 发表于：Genome biology · 年份：2010 · DOI：10.1186/gb-2010-11-12-r119 · 被引用次数：628 · 研究领域：CRISPR and Genetic Engineering、Genomics and Phylogenetic Studies、Chromosomal and Genetic Variations

We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing.