Characterization of CD45 and CD45R monoclonal antibodies using transfected mouse cell lines that express individual human leukocyte common antigens.

作者：Michel Streuli, Chikao Morimoto, M Schrieber, Stuart F. Schlossman, Hirohisa Saito · 发表于：The Journal of Immunology · 年份：1988 · DOI：10.4049/jimmunol.141.11.3910 · 被引用次数：137 · 研究领域：Monoclonal and Polyclonal Antibodies Research、Cell Adhesion Molecules Research、T-cell and B-cell Immunology

Five cell lines that express one of five isoforms of the human leukocyte common Ag (CD45) were established by transfecting a murine pre-B cell line with leukocyte common Ag cDNA constructs. Using these cell lines, the specificities of CD45 and CD45R mAb were examined. Of the 43 mAb tested, 25 antibodies recognized sequences common to all five isoforms, 16 antibodies recognized isoforms that include exon A encoded sequences, one antibody recognized isoforms that include exon B encoded sequences, and one antibody recognized only the isoform that does not include either exon A, B, or C encoded sequences.