Putative resistance gene markers associated with quantitative trait loci for fire blight resistance in Malus ‘Robusta 5’ accessions

作者：Susan E. Gardiner, John L. Norelli, Nihal De Silva, Gennaro Fazio, Andreas Peil, Mickaël Malnoy, Mary B. Horner, Deepa R. Bowatte, Charmaine Carlisle, Claudia Wiedow, Yizhen Wan, Carole Leavel Bassett, Angela M. Baldo, Jean‐Marc Celton, Klaus Richter, Herb S. Aldwinckle, Vincent G. M. Bus · 发表于：BMC Genetics · 年份：2012 · DOI：10.1186/1471-2156-13-25 · 被引用次数：101 · 研究领域：Plant Pathogenic Bacteria Studies、Fungal Plant Pathogen Control、Postharvest Quality and Shelf Life Management

BACKGROUND: Breeding of fire blight resistant scions and rootstocks is a goal of several international apple breeding programs, as options are limited for management of this destructive disease caused by the bacterial pathogen Erwinia amylovora. A broad, large-effect quantitative trait locus (QTL) for fire blight resistance has been reported on linkage group 3 of Malus 'Robusta 5'. In this study we identified markers derived from putative fire blight resistance genes associated with the QTL by integrating further genetic mapping studies with bioinformatics analysis of transcript profiling data and genome sequence databases. RESULTS: When several defined E.amylovora strains were used to inoculate three progenies from international breeding programs, all with 'Robusta 5' as a common parent, two distinct QTLs were detected on linkage group 3, where only one had previously been mapped. In the New Zealand 'Malling 9' X 'Robusta 5' population inoculated with E. amylovora ICMP11176, the proximal QTL co-located with SNP markers derived from a leucine-rich repeat, receptor-like protein (MxdRLP1) and a closely linked class 3 peroxidase gene. While the QTL detected in the German 'Idared' X 'Robusta 5' population inoculated with E. amylovora strains Ea222_JKI or ICMP11176 was approximately 6 cM distal to this, directly below a SNP marker derived from a heat shock 90 family protein gene (HSP90). In the US 'Otawa3' X 'Robusta5' population inoculated with E. amylovora strains Ea273 or E2002...