HLA‐D‐restricted Antigen Activation of Sensitized T Lymphocytes: Studies on the Ability of HLA‐D/DR‐expressing B Lymphocytes to Substitute for Macrophages in Antigen Activation

作者：B. O. BERGHOLTZ, Erik Thorsby · 发表于：Scandinavian Journal of Immunology · 年份：1979 · DOI：10.1111/j.1365-3083.1979.tb01349.x · 被引用次数：18 · 研究领域：Immunotherapy and Immune Responses、Immune Cell Function and Interaction、T-cell and B-cell Immunology

The proliferative response of sensitized human T lymphocytes to purified protein derivative (PPD) and to trinitrophenyl (TNP)-conjugated autologous cells in vitro is restricted by self HLA-D/DR determinants. Here we report that the PPD-specific response is strictly related to the content of phagocytosing cells (macrophages, Mphi) in the cultures and that an optimal PPD response occurred at a T/Mphi ratio between 10:1 and 5:1. B-cell-enriched suspensions, which also express the HLA-D/DR determinants, were not able to replace the macrophages in this HLA-D/DR-restricted response. On the other hand, TNP-treated similarly prepared B cells were in most instances effective in inducing a secondary TNP-specific response of in vitro-sensitized T cells.