A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.

作者：Nancy K. Dwyer, G Blobel · 发表于：The Journal of Cell Biology · 年份：1976 · DOI：10.1083/jcb.70.3.581 · 被引用次数：423 · 研究领域：Nuclear Structure and Function、RNA Research and Splicing、Metabolism and Genetic Disorders

A modified procedure for the isolation of a nuclear pore complex-lamina fraction from rat liver nuclei is described. Evidence is provided that the isolated lamina, a 150-A thick, proteinaceous structure, apposes the inner nuclear envelope membrane, connecting nuclear pore complexes and surrounding the entire nucleus.