Identification of Genes Involved in Cytochrome c Biogenesis in Shewanella oneidensis , Using a Modified mariner Transposon

作者：Rachida Bouhenni, Andrew R. Gehrke, Daâd A. Saffarini · 发表于：Applied and Environmental Microbiology · 年份：2005 · DOI：10.1128/aem.71.8.4935-4937.2005 · 被引用次数：101 · 研究领域：Microbial Fuel Cells and Bioremediation、Microbial Community Ecology and Physiology、Protist diversity and phylogeny

A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon insertions in the cytochrome c maturation genes ccmC and ccmF1, respectively, were analyzed. Both mutants were deficient in anaerobic respiration and cytochrome c production.