CD1d-Restricted and TCR-Mediated Activation of V α 14 NKT Cells by Glycosylceramides

作者：Tetsu Kawano, Junqing Cui, Yasuhiko Koezuka, Isao Toura, Yoshikatsu Kaneko, Kazuhiro Motoki, Hitomi Ueno, Ryusuke Nakagawa, Hiroshi Sato, Eisuke Kondo, Haruhiko Koseki, Masaru Taniguchi · 发表于：Science · 年份：1997 · DOI：10.1126/science.278.5343.1626 · 被引用次数：2410 · 研究领域：Immune Cell Function and Interaction、Glycosylation and Glycoproteins Research、Immune Response and Inflammation

Natural killer T (NKT) lymphocytes express an invariant T cell antigen receptor (TCR) encoded by the Valpha14 and Jalpha281 gene segments. A glycosylceramide-containing alpha-anomeric sugar with a longer fatty acyl chain (C26) and sphingosine base (C18) was identified as a ligand for this TCR. Glycosylceramide-mediated proliferative responses of Valpha14 NKT cells were abrogated by treatment with chloroquine-concanamycin A or by monoclonal antibodies against CD1d/Vbeta8, CD40/CD40L, or B7/CTLA-4/CD28, but not by interference with the function of a transporter-associated protein. Thus, this lymphocyte shares distinct recognition systems with either T or NK cells.