Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing

作者：Andrew Adey, Jay Shendure · 发表于：Genome Research · 年份：2012 · DOI：10.1101/gr.136242.111 · 被引用次数：135 · 研究领域：Epigenetics and DNA Methylation、RNA modifications and cancer、Tracheal and airway disorders

We have adapted transposase-based in vitro shotgun library construction ("tagmentation") for whole-genome bisulfite sequencing. This method, Tn5mC-seq, enables a >100-fold reduction in starting material relative to conventional protocols, such that we generate highly complex bisulfite sequencing libraries from as little as 10 ng of input DNA, and ample useful sequences from 1 ng of input DNA. We demonstrate Tn5mC-seq by sequencing the methylome of a human lymphoblastoid cell line to ∼8.6× high-quality coverage of each strand.