Characterization of Yersinia enterocolitica and Yersinia pseudotuberculosis by Deoxyribonucleic Acid Hybridization and by Biochemical Reactions

作者：D J Brenner, Arnold G. Steigerwalt, Deise Pasetto Falcão, Robert E. C. Weaver, George R. Fanning · 发表于：International Journal of Systematic Bacteriology · 年份：1976 · DOI：10.1099/00207713-26-2-180 · 被引用次数：127 · 研究领域：Yersinia bacterium, plague, ectoparasites research、Pharmacological Effects of Natural Compounds、Plant-based Medicinal Research

Strains of Yersinia enterocolitica and Yersinia pseudotuberculosis were characterized by deoxyribonucleic acid (DNA) hybridization, and the extent of DNA relatedness between the strains was assessed by hydroxyapatite chromatography. DNAs from 24 strains of Y. pseudotuberculosis were highly related, but Y. pseudotuberculosis was only 40 to 60% related to Y. enterocolitica. Y. enterocolitica strains formed three DNA relatedness groups, and the data strongly suggest a fourth relatedness group. Each relatedness group can be defined biochemically. One DNA relatedness group corresponds to typical Y. enterocolitica; the second is rhamnose positive; the third is rhamnose positive, melibiose positive, a-methyl glucoside positive, and raffinose positive. The suggested fourth hybridization group is sucrose negative. Although all of these DNA relatedness groups should remain in the genus Yersinia, only the first group is Y. enterocolitica. All of the yersiniae tested are distantly, but significantly, related to other members of Enterobacteriaceae. Yersiniae were more related to members of Enterobacteriaceae than to Pasteurella multocida.