Kinetics of gas diffusion in mammalian cell culture systems. I. Experimental

作者：William F. McLimans, Elizabeth J. Crouse, Kenneth V. Tunnah, George E. Moore · 发表于：Biotechnology and Bioengineering · 年份：1968 · DOI：10.1002/bit.260100603 · 被引用次数：48 · 研究领域：3D Printing in Biomedical Research、Pluripotent Stem Cells Research、Mesenchymal stem cell research

Abstract It has been demonstrated experimentally that the thickness of fluid overlay in conventional tissue culture systems limits the oxygen available to mammalian cells growing as a submerged monolayer. A rocker culture system is described which circumvents critical problems associated with thin film culture while permitting nearly unlimited access of oxygen to the cell monolayer. Good growth of primary hepatic cells as isolated sheets has been obtained.