Cell immersion and cell dipping in microfluidic devicesElectronic supplementary information (ESI) available: cell dipping video sequence from which Fig. 7 was extracted and cell dipping video sequence with close-ups. See http://www.rsc.org/suppdata/lc/b3/b311210a/

作者：Urban Seger, Shady Gawad, Robert M. Johann, Arnaud Bertsch, Philippe Renaud · 发表于：Lab on a Chip · 年份：2004 · DOI：10.1039/b311210a · 被引用次数：83 · 研究领域：Microfluidic and Bio-sensing Technologies、Electrowetting and Microfluidic Technologies、Electrohydrodynamics and Fluid Dynamics

Combining deflective dielectrophoretic barriers with controlled pressure driven liquid flows in microfluidic devices allows accurate handling of particles such as biological cells in suspensions. Working towards cell-based lab-on-a-chip applications, a platform permitting rapid testing of devices having different dielectrophoretic and fluidic subunits was developed. The performance of such a system is shown in the cases of (A) flooding a small number of immobilised cells with a dye and (B) transient buffer swapping of a large number of cells in flow. The transition times for moving cells from one reagent to the other are below 0.5 s in the case of flow-through cell dipping.