Visualized analysis of cellular fatty acid profiles ofVibrio parahaemolyticusstrains under cold stress

作者：Juntao Jia, Ying Chen, Yinghui Jiang, Jing Tang, Lijun Yang, Chengzhu Liang, Zhen Jia, Liqing Zhao · 发表于：FEMS Microbiology Letters · 年份：2014 · DOI：10.1111/1574-6968.12498 · 被引用次数：20 · 研究领域：Vibrio bacteria research studies、Metabolomics and Mass Spectrometry Studies、Advanced Chemical Sensor Technologies

Vibrio parahaemolyticus is a common foodborne bacterial pathogen, which survives in cold environments and is sometimes difficult to culture. Fatty acid analysis under cold stress was conducted for several V. parahaemolyticus strains using gas chromatography/mass spectrometry, and the results were compared with those of the controls. All the fatty acid profiles obtained were visualized by multidimensional scaling (MDS) and self-organized map (SOM). It was observed that the fatty acid profiles of V. parahaemolyticus substantially changed under cold stress. The percentage of methyl palmitate remarkably decreased and that of methyl palmitoleate (except for two strains) and methyl oleate increased. These findings demonstrate the role of fatty acids in cold stress. The changes in the fatty acid profiles illustrated by MDS and SOM could differentiate strains under cold stress from the controls and can potentially lead to a method of detecting injured cold-stressed V. parahaemolyticus.