Establishment of a Rat Model of Type II Diabetic Neuropathic Pain

作者：Jiang-kun Dang, Yan Wu, Hong Cao, Bo Meng, Cong‐Cong Huang, Guo Chen, Jun Li, Xue‐Jun Song, Qingquan Lian · 发表于：Pain Medicine · 年份：2014 · DOI：10.1111/pme.12387_1 · 被引用次数：47 · 研究领域：Pain Mechanisms and Treatments、Spine and Intervertebral Disc Pathology、Pain Management and Treatment

OBJECTIVE: To establish a rat model of type II diabetic neuropathic pain. METHODS: Sixty Sprague Dawley rats were randomly divided into two groups: group A (N = 10) was fed a normal diet, and group B (N = 50) was fed a high-fat and high-sugar diet. After 8 weeks, the body weight of all rats was recorded, and rats in both groups had their fasting plasma glucose, insulin concentration, and insulin sensitivity index measured and calculated. Subsequently, the rats in group B were randomly divided into three subgroups that were each given different doses of streptozotocin (STZ) by a single intraperitoneal injection (subgroup B1 received 30 mg/kg, subgroup B2 received 35 mg/kg, and subgroup B3 40 mg/kg). Two weeks after the STZ injection, the four groups of rats had their insulin sensitivity index, mechanical withdrawal threshold, and thermal withdrawal latency assessed, allowing us to establish a rat model of type II diabetic neuropathic pain and to determine the optimum dose of STZ. Four weeks after STZ injection (2 weeks after the model was established), the pain threshold was measured in the rats in group A and the group treated with the most effective STZ dose. We also measured the expression of phosphorylated extracellular signal-regulated kinase (p-ERK), phosphorylated cyclic AMP response element-binding protein (p-CREB), and phosphorylated N-methyl d-aspartate receptor subtype B (p-NR2B) in the dorsal root ganglion (DRG) and spinal cord dorsal horn regions, which are closel...