Transcriptional Control Signals of a Eukaryotic Protein-Coding Gene

作者：Steven Lanier McKnight, Robert C. Kingsbury · 发表于：Science · 年份：1982 · DOI：10.1126/science.6283634 · 被引用次数：1397 · 研究领域：Heat shock proteins research、Viral Infectious Diseases and Gene Expression in Insects、Insect and Pesticide Research

Transcriptional control signals of a model eukaryotic protein-coding gene have been identified by a new procedure of in vitro mutagenesis. This method allows small clusters of nucleotide residues to be substituted in a site-directed manner without causing the addition or deletion of other sequences. Transcription assays of a systematic series of these clustered point mutants have led to the identification of three distinct control signals located within the 105-nucleotide residues immediately upstream from the point where transcription begins.