Tumor-Associated Macrophages: The Double-Edged Sword in Cancer Progression

作者：Jeremy J.W. Chen, Yi‐Chen Lin, Pei‐Li Yao, Ang Yuan, Hsang-Yu Chen, Chia‐Tung Shun, Meng‐Feng Tsai, Chun‐Houh Chen, Pan‐Chyr Yang · 发表于：Journal of Clinical Oncology · 年份：2004 · DOI：10.1200/jco.2005.12.172 · 被引用次数：377 · 研究领域：Immune cells in cancer、Protease and Inhibitor Mechanisms、Chemokine receptors and signaling

PURPOSE: Inflammation plays a critical role in cancer progression. In this study we investigate the pro-tumorigenic activities and gene expression profiles of lung cancer cells after interaction with macrophages. MATERIALS AND METHODS: We measured intratumoral microvessel counts and macrophage density in 41 lung cancer tumor specimens and correlated these with the patients' clinical outcome. The interaction between macrophages and cancer cell lines was assessed using a transwell coculture system. The invasive potential was evaluated by in vitro invasion assay. The matrix-degrading activity was assayed by gelatin zymography. The microarray was applied to a large-scale analysis of the genes involved in the interaction, as well as to monitor the gene expression profiles of lung cancer cells responding to anti-inflammatory drugs in cocultures. RESULTS: The macrophage density positively correlated with microvessel counts and negatively correlated with patient relapse-free survival (P < .05). After coculture with macrophages, lung cancer cell lines exhibited higher invasive potentials and matrix-degrading activities. We identified 50 genes by microarray that were upregulated more than two-fold in cancer cells after coculture. Northern blot analyses confirmed some gene expression such as interleukin-6, interleukin-8, and matrix metalloproteinase 9. The two-dimensional hierarchical clustering also demonstrated that the gene expression profiles of lung cancer cells responding to vario...