Elevated relative fluorescence intensity of CD38 antigen expression on CD8+ T cells is a marker of poor prognosis in HIV infection: Results of 6 years of follow-up

作者：Zhiyuan Liu, Lance E. Hultin, William G. Cumberland, Patricia M. Hultin, Ingrid Schmid, Jose L. Matud, Roger Detels, Janis V. Giorgi · 发表于：Cytometry · 年份：1996 · DOI：10.1002/(sici)1097-0320(19960315)26:1<1::aid-cyto1>3.0.co;2-l · 被引用次数：141 · 研究领域：Cytomegalovirus and herpesvirus research、Calcium signaling and nucleotide metabolism、Immune Cell Function and Interaction

Relative fluorescence intensity measurements from a flow cytometer were used to evaluate expression of CD38 and HLA-DR antigens. These molecules are associated with cellular activation and are present at increased levels on the CD8+ lymphocytes of HIV-infected subjects. In the current study, the prognostic value of mean fluorescence intensity measurements of CD38 and HLA-DR on CD8+ cells was compared to results from our previous study in which we reported prognostic value for an elevated percentage of CD8+ cells that were positive for expression of the CD38 antigen (Giorgi et al.: JAIDS 6:904-912, 1993). Using the proportional hazards model, elevated mean fluorescence intensity of CD38 expression on CD8+ cells had prognostic value for development of AIDS that was almost identical to the prognostic value of the percentage of CD8+ cells that were positive for expression of CD38. This prognostic value was in addition to that provided by the patient's CD4+ cell measurement. To our knowledge, this is the first report that a measurement of fluorescence intensity can be used as a prognostic marker in an immunodeficiency disease. Efforts are needed to establish methods that will allow widespread application of this observation in the clinical management of HIV-infected subjects.