Transcription initiation complexes and upstream activation with RNA polymerase II lacking the C-terminal domain of the largest subunit.

作者：Stephen Buratowski, Phillip A. Sharp · 发表于：Molecular and Cellular Biology · 年份：1990 · DOI：10.1128/mcb.10.10.5562 · 被引用次数：53 · 研究领域：Virus-based gene therapy research、Genomics and Chromatin Dynamics、Animal Genetics and Reproduction

RNA polymerase II assembles with other factors on the adenovirus type 2 major late promoter to generate pairs of transcription initiation complexes resolvable by nondenaturing gel electrophoresis. The pairing of the complexes is caused by the presence or absence of the C-terminal domain of the largest subunit. This domain is not required for transcription stimulation by the major late transcription factor in vitro.