Nuctease digestion promotes structural rearrangements m H1-depleted chromatin

作者：Wolfgang O. Weischet, K. E. Van Holde · 发表于：Nucleic Acids Research · 年份：1980 · DOI：10.1093/nar/8.17.3743 · 被引用次数：16 · 研究领域：Enzyme Structure and Function、DNA and Nucleic Acid Chemistry、Polyamine Metabolism and Applications

Digestion of H1-depleted chromatin with micrococcal nuclease at an ionic strength of 0.35M gives rise to structural rearrangements indicating nucleosomal sliding. The ionic strength necessary to reveal this effect is significantly lower than that required in the absence of an accompanying digestion. As an explanation, a model is presented in which the progressing terminal degradation of oligomeric nucleosomes is made responsible for promoting structural rearrangements.