RAPID REGENERATION OF DRACOCEPHALUM KOTSCHYI BOISS. FROM NODAL EXPLANTS

作者：Kosar Moradi, Mahmoud Otroshy · 发表于：International Journal of Life Science and Medical Research · 年份：2013 · DOI：10.5963/lsmr0301002 · 被引用次数：7 · 研究领域：Plant tissue culture and regeneration、Fern and Epiphyte Biology、Plant Pathogens and Fungal Diseases

The Dracocephalum Kotschyi L., a wild-growing flowering plant belonging to the family Lamiaceae is best known for the essential oils common to many members of the family. The present study showed the procedure for propagation of D. kotschyi using nodal segments from in vitro germinated plants. A high frequency and rapid regeneration protocol was growing and developing from nodal explants of D. kotschyi on the medium MS containing 2mg/L BAP plus 0.5mg/L NAA. Histochemical analysis showed a direct induction of more teratological protuberances that arise around the cut end of the explants. After hardening, the rooted plants were transferred to the greenhouse condition where they normally grew, matured and flowered with a survival rate of 90-95%. We concluded that the present protocol can be efficiently used for mass propagation of D. kotschyi.