Critical Stages of Extracting DNA from Aspergillus fumigatus in Whole-Blood Specimens

作者：Philip Lewis White, Michael D. Perry, Juergen Loeffler, Willem J. G. Melchers, Lena Klingspor, Stéphane Bretagne, Elaine McCulloch, Manuel Cuenca‐Estrella, Niklas Finnström, J. Peter Donnelly, Rosemary A. Barnes · 发表于：Journal of Clinical Microbiology · 年份：2010 · DOI：10.1128/jcm.01466-10 · 被引用次数：89 · 研究领域：Antifungal resistance and susceptibility、Fungal Infections and Studies、Mycotoxins in Agriculture and Food

A standardized protocol for extracting DNA from Aspergillus fumigatus has been proposed by the European Aspergillus PCR Initiative (EAPCRI). Using meta-regression analysis, the EAPCRI showed certain stages of the process to be critical to providing a satisfactory analytical sensitivity. The study investigated each step of the EAPCRI protocol by elimination and monitored the influence on Aspergillus PCR performance.