Gene expression profiling of skeletal muscle in exercise-trained and sedentary rats with inborn high and low VO2max

作者：Anja Bye, Morten A. Høydal, Daniele Catalucci, Mette Langaas, Ole Johan Kemi, Vidar Beisvåg, Lauren G. Koch, Steven L. Britton, Øyvind Ellingsen, Ulrik Wisløff · 发表于：Physiological Genomics · 年份：2008 · DOI：10.1152/physiolgenomics.90282.2008 · 被引用次数：40 · 研究领域：Adipose Tissue and Metabolism、Muscle metabolism and nutrition、Mitochondrial Function and Pathology

The relationship between inborn maximal oxygen uptake (VO2max) and skeletal muscle gene expression is unknown. Since low VO2maxis a strong predictor of cardiovascular mortality, genes related to low VO2maxmight also be involved in cardiovascular disease. To establish the relationship between inborn VO2maxand gene expression, we performed microarray analysis of the soleus muscle of rats artificially selected for high- and low running capacity (HCR and LCR, respectively). In LCR, a low VO2maxwas accompanied by aggregation of cardiovascular risk factors similar to the metabolic syndrome. Although sedentary HCR were able to maintain a 120% higher running speed at VO2maxthan sedentary LCR, only three transcripts were differentially expressed (FDR ≤ 0.05) between the groups. Sedentary LCR expressed high levels of a transcript with strong homology to human leucyl-transfer RNA synthetase, of whose overexpression has been associated with a mutation linked to mitochondrial dysfunction. Moreover, we studied exercise-induced alterations in soleus gene expression, since accumulating evidence indicates that long-term endurance training has beneficial effects on the metabolic syndrome. In terms of gene expression, the response to exercise training was more pronounced in HCR than LCR. HCR upregulated several genes associated with lipid metabolism and fatty acid elongation, whereas LCR upregulated only one transcript after exercise training. The results indicate only minor differences in sole...