Crystallographic structure analysis of lamprey hemoglobin from anomalous dispersion of synchrotron radiation

作者：Wayne A. Hendrickson, Janet L. Smith, R. Paul Phizackerley, Ethan A. Merritt · 发表于：Proteins Structure Function and Bioinformatics · 年份：1988 · DOI：10.1002/prot.340040202 · 被引用次数：93 · 研究领域：Hemoglobin structure and function、Protein Structure and Dynamics、Enzyme Structure and Function

The molecular structure of lamprey hemoglobin was previously determined and refined by conventional crystallographic analysis. In this study, the structural analysis has been repeated in the course of developing the method of multiwavelength anomalous diffraction (MAD) for phase determination. New experimental and analytical procedures that were devised to perform this determination should have general applicability. These include an experimental design to optimize signal strength and reduce systematic errors, experimental evaluation of anomalous scattering factors, and a least-squares procedure for analyzing the MAD data. MAD phases for the structure at 3 A resolution are as accurate overall as the multiple isomorphous replacement (MIR) phases determined previously.