Non‐degradative dissolution and acetylation of ball‐milled plant cell walls: high‐resolution solution‐state NMR

作者：Fachuang Lu, John Ralph · 发表于：The Plant Journal · 年份：2003 · DOI：10.1046/j.1365-313x.2003.01817.x · 被引用次数：346 · 研究领域：Lignin and Wood Chemistry、Fermentation and Sensory Analysis、Plant Gene Expression Analysis

Two solvent systems for fully dissolving, and optionally derivatizing, finely ground plant cell wall material at room temperature are described: dimethylsulfoxide (DMSO) and tetrabutylammonium fluoride (TBAF) or N-methylimidazole (NMI). In situ acetylation produces acetylated cell walls (Ac-CWs) that are fully soluble in chloroform. Lignin structures tested remain fully intact. The dispersion of 13C-1H correlations afforded by two-dimensional (2D) nuclear magnetic resonance (NMR) experiments reveals the major lignin units, allowing the whole lignin fraction to be analyzed by high-resolution solution-state NMR methods for the first time. Non-degradative cell wall dissolution offers the potential to analyze polysaccharide components, and improve current cell wall analytical methods by using standard homogeneous solution-state chemistry.