Multidirectional chromosome painting substantiates the occurrence of extensive genomic reshuffling within Accipitriformes

作者：Wenhui Nie, Patricia C. O’Brien, Beiyuan Fu, Jinghuan Wang, Weiting Su, Kai He, Bertrand Bed’Hom, V. Volobouev, M.A. Ferguson‐Smith, Gauthier Dobigny, Fengtang Yang · 发表于：BMC Evolutionary Biology · 年份：2015 · DOI：10.1186/s12862-015-0484-0 · 被引用次数：23 · 研究领域：Chromosomal and Genetic Variations、Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities、Genomic variations and chromosomal abnormalities

BACKGROUND: Previous cross-species painting studies with probes from chicken (Gallus gallus) chromosomes 1-10 and a paint pool of nineteen microchromosomes have revealed that the drastic karyotypic reorganization in Accipitridae is due to extensive synteny disruptions and associations. However, the number of synteny association events and identities of microchromosomes involved in such synteny associations remain undefined, due to the lack of paint probes derived from individual chicken microchromosomes. Moreover, no genome-wide homology map between Accipitridae species and other avian species with atypical karyotype organization has been reported till now, and the karyotype evolution within Accipitriformes remains unclear. RESULTS: To delineate the synteny-conserved segments in Accipitridae, a set of painting probes for the griffon vulture, Gyps fulvus (2n = 66) was generated from flow-sorted chromosomes. Together with previous generated probes from the stone curlew, Burhinus oedicnemus (2n = 42), a Charadriiformes species with atypical karyotype organization, we conducted multidirectional chromosome painting, including reciprocal chromosome painting between B. oedicnemus and G. fulvus and cross-species chromosome painting between B. oedicnemus and two accipitrid species (the Himalayan griffon, G. himalayensis 2n = 66, and the common buzzard, Buteo buteo, 2n = 68). In doing so, genome-wide homology maps between B. oedicnemus and three Accipitridae species were established. F...