A Sampling of the Yeast Proteome

作者：Bruce Futcher, Gerald I. Latter, Pat J. Monardo, C S McLaughlin, James I. Garrels · 发表于：Molecular and Cellular Biology · 年份：1999 · DOI：10.1128/mcb.19.11.7357 · 被引用次数：620 · 研究领域：Fungal and yeast genetics research、Advanced Proteomics Techniques and Applications、Microbial Metabolic Engineering and Bioproduction

In this study, we examined yeast proteins by two-dimensional (2D) gel electrophoresis and gathered quantitative information from about 1,400 spots. We found that there is an enormous range of protein abundance and, for identified spots, a good correlation between protein abundance, mRNA abundance, and codon bias. For each molecule of well-translated mRNA, there were about 4,000 molecules of protein. The relative abundance of proteins was measured in glucose and ethanol media. Protein turnover was examined and found to be insignificant for abundant proteins. Some phosphoproteins were identified. The behavior of proteins in differential centrifugation experiments was examined. Such experiments with 2D gels can give a global view of the yeast proteome.