Glutathione-complexed iron–sulfur clusters. Reaction intermediates and evidence for a template effect promoting assembly and stability

作者：Wenbin Qi, Jingwei Li, Cecilia Y. Chain, G. A. Pasquevich, A. F. Pasquevich, J. A. Cowan · 发表于：Chemical Communications · 年份：2013 · DOI：10.1039/c3cc43620a · 被引用次数：30 · 研究领域：Metalloenzymes and iron-sulfur proteins、Metal-Catalyzed Oxygenation Mechanisms、Metal complexes synthesis and properties

Assembly and stabilization of a glutathione-complexed [2Fe-2S] cluster is promoted by aggregation of glutathione. The cluster core selects the tetramer species from a collection of equilibrating solution aggregate species, and in turn the core is stabilized toward hydrolytic degradation. Studies of glutathione derivatives, in combination with mass spectrometric and Mössbauer investigations provide insight on reaction intermediates during formation of [2Fe-2S](GS)4(2-).