Molecular structure of the cell wall receptor for killer toxin KT28 in Saccharomyces cerevisiae

作者：Manfred Schmitt, Ferdinand Radler · 发表于：Journal of Bacteriology · 年份：1988 · DOI：10.1128/jb.170.5.2192-2196.1988 · 被引用次数：70 · 研究领域：Fungal and yeast genetics research、Toxin Mechanisms and Immunotoxins、Plant Reproductive Biology

The adsorption of the yeast killer toxin KT28 to susceptible cells of Saccharomyces cerevisiae was prevented by concanavalin A, which blocks the mannoprotein receptor. Certain mannoprotein mutants of S. cerevisiae that lack definite structures in the mannan of their cell walls were found to be resistant to KT28, whereas the wild-type yeast from which the mutants were derived was susceptible. Isolated mannoprotein from a resistant mutant was unable to adsorb killer toxin. By comparing the resistances of different mannoprotein mutants, information about the molecular structure of the receptor was obtained. At least two mannose residues have to be present in the side chains of the outer chain of the cell wall mannan, whereas the phosphodiester-linked mannose group is not essential for binding and the subsequent action of killer toxin KT28.